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Home > ABOUT THE INSTITUTE > Organization > EMI > Laboratory for Electron Microscopy and Diagnostics of Gastroenteric Viruses

Laboratory for Electron Microscopy and Diagnostics of Gastroenteric Viruses

A Brief Description of the Laboratory

The laboratory deals with the direct and indirect identification of viruses in various clinical samples, such as excrements, skin vesicles, crusts, urine, animal and human organs or tissue and cell cultures. The main focus of the laboratory tasks is on diagnostics ofgastroenteritis viruses- rotaviruses, adenoviruses, astroviruses, caliciviruses (noroviruses and sapoviruses) and coronaviruses. The laboratory has significantly contributed to the developments of immunocytochemical methods using colloid gold for determining viral antigens in transmission electron microscopy.

The laboratory work comprises also carrying out of enzyme immunoassays for detecting rotavirus and , enteric adenovirus antigens  andcalicivirus (norovirus, sapovirus) and astrovirus nucleid acids by using most updated molecular methods.

The routine work was expanded to cover also detection of enteric viruses in water and other environmental samples as well as in food.

The laboratory is included in the European network of electron microscopy laboratories specialized in virus diagnostics "External Quality Assurance, Electron Microscopy Virus Diagnostics  (EQA-EMV)", in the program "Labquality, External Quality Assessment" for rotavirus and adenovirus diagnostics, in the European rotavirus genotyping network "EuroRotaNet", and in "NoroNet" - the European norovirus genotyping network.

The research work of the laboratory is closely intertwined with its professional work. In the preceding years, the laboratory was included in the project of the Sixth Framework Programme ″EU - STREP – EVENT, Enteric virus emergence, new tools" laying a special stress on food- and water-transmitted viruses. The laboratory is participating in or is conducting a number of different projects which are carried out with the aim of explaining the etiological role of individual pathogens in gastroenteritis, with a special regard to enteritis viruses. Therefore, in-depth investigations of molecular and epidemiological properties of rotaviruses and noroviruses, as well as their genetic diversity and zoonotic potential are conducted.

The research team is specialized in identifying virus morphology and ultrastructure by using conventional room temperature embedding and and freeze substitution methods in transmission electron microscopy. The laboratory was the first in Slovenia to use cryo methods for viral and bacterial suspensions and for virus- or bacteria-infected cells or tissues.

The laboratory also participates in education program. Students, residents and junior research fellows have access to the laboratory where they have the opportunity to get acquainted with the basics of electron microscopy and its role in rapid diagnostics of viral infections.

Phone:

  • +386 1 543 74 62

Head of the Laboratory:

Other Staff:

  • Andrej Steyer, BSc (Microbiol.), PhD, Research Associate
  • Marko Kolenc, BSc (Microbiol.)
  • Irena Šest

CONTENT

Organizational Structure of the Institute of Microbiology and Immunology

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IN NUMBERS

NEW DIAGNOSTIC TESTS

17

in the year 2012

13

in the year 2013

15

in the year 2014

OUR POTENTIAL

8

professors

26

doctors of science

120

employees

PUBLISHED SCIENTIFIC PAPERS – SCI

46

in the year 2013

49

in the year 2014

88

in the year 2015

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